Single Nucleotides Polymorphisms in COX2 Gene and their Association with Signs and Symptoms of Teething - A Pilot Study

ABSTRACT Objective: To investigate the association between single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) and local and systemic signs and symptoms of teething. Material and Methods: Forty-four pairs of mothers-babies/toddlers were included. Erupted primary teeth were evaluated during clinical examination. Local and systemic signs and symptoms of teething were obtained from mothers' reporting via anamnesis. Samples of buccal cells were retrieved for DNA genotyping using real-time PCR. The T-test, Chi-square test, logistic regression, and haplotype analyses were applied. Results: Almost all mothers (95.5%) reported at least one local or systemic sign and symptom of teething. The most common was increased salivation (79.5%), diarrhea (72.3 %), and fever (70.5 %). The mean number of signs and symptoms per child was higher in boys than girls (mean = 5.1; SD= 1.5; p=0.008). Sleep disturbance (p=0.03) and loss of appetite (p=0.05) were more reported in boys. The rs689466 and rs5275 were not associated with signs and symptoms of teething (p>0.05). Conclusion: The single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) were not associated with local and systemic signs and symptoms of teething.

Potential interactions among single nucleotide polymorphisms in bone- and cartilage-related genes in skeletal malocclusions.

OBJECTIVE: To investigate SNPs in bone- and cartilage-related genes and their interaction in the aetiology of sagittal and vertical skeletal malocclusions. SETTINGS AND SAMPLE POPULATION: This study included 143 patients and classified as follows: skeletal class I (n = 77), class II (n = 47) and class III (n = 19); maxillary retrusion (n = 39), protrusion (n = 52) and well-positioned maxilla (n = 52); mandibular retrognathism (n = 50), prognathism (n = 50) and well-positioned mandible (n = 43); normofacial (n = 72), dolichofacial (n = 55) and brachyfacial (n = 16). MATERIALS AND METHODS: Steiner's ANB, SNA, SNB angles and Ricketts' NBa-PtGn angle were measured to determine the skeletal malocclusion and the vertical pattern. Nine SNPs in BMP2, BMP4, SMAD6, RUNX2, WNT3A and WNT11 were genotyped. Chi-squared test was used to compare genotypes among the groups. Multifactor dimensionality reduction (MDR) and binary logistic regression analysis, both using gender and age as co-variables, were also used. We performed Bonferroni correction for multiple testing. RESULTS: Significant associations at P < .05 were observed for SNPs rs1005464 (P = .042) and rs235768 (P = .021) in BMP2 with mandibular retrognathism and for rs59983488 (RUNX2) with maxillary protrusion (P = .04) as well as for rs708111 (WNT3A) with skeletal class III (P = .02; dominant model), rs1533767 (WNT11) with a brachyfacial skeletal pattern (P = .01, OR = 0.10; dominant model) and for rs3934908 (SMAD6) with prognathism (P = .02; recessive model). After the Bonferroni correction, none of the SNPs remained associated. The MDR predicted some interaction for skeletal class II, dolichofacial and brachyfacial phenotypes. CONCLUSION: Our results suggest that SNPs in BMP2, BMP4, SMAD6, RUNX2, WNT3A and WNT11 could be involved in the aetiology of sagittal and vertical malocclusions.

Genetic polymorphisms in interleukin-6 and interleukin-1-beta were associated with dental caries and gingivitis.

OBJECTIVE: Evaluate the association between single nucleotide polymorphisms (SNPs) in Interleukin-6 (IL-6) gene (rs1800795) and in Interleukin-1-beta (IL-1ß) gene (rs1143627 and rs1143629) with dental caries and gingivitis in Brazilian children. MATERIAL AND METHODS: Three hundred and fifty-three children aged 8-11 years were included. Visible biofilm and gingival bleeding were evaluated by Community Periodontal Index. The International System for Detection and Assessment of Carious Lesions (ICDAS) was used to investigate dental caries. Real-time PCR evaluated SNPs in the DNA. Chi-square test, haplotype analysis and logistic regression were applied (alpha of 5%). RESULTS: The GG genotype in rs1800795 (IL-6) decreases the risk of gingivitis in a co-dominant model (p = .05; OR = 0.64). The GG genotype in rs1143627 (IL-1ß) reduces the risk of dental caries (Co-dominant model: ICDAS0 versus ICDAS1-6: p = .05; OR = 0.55. ICDAS0-2 versus ICDAS3-6: p = .02; OR = 0.49. Recessive model: ICDAS0 versus ICDAS1-6: p = .005; OR = 0.48. ICDAS0-2 versus ICDAS3-6: p = .004; OR = 0.45. Logistic regression: ICDAS0-2 versus ICDAS3-6: p = .05; OR = 0.24; CI 95%= 0.05-1.00). The GG genotype in rs1143629 was more frequent in ICDAS0 (p = .05; OR: 0.60). In the haplotype analysis, IL-1ß was associated with gingivitis. CONCLUSION: The rs1800795 in IL-6 gene was associated with gingivitis. The rs1143627 and rs1143629 in IL-1ß were associated with dental caries and gingivitis.

Fratura de implante ancorado no assoalho nasal / Fracture nasal floor implant - a case report

A reabilitação protética com implantes osseointegrados é uma realidade atual. Os implantes contemporâneos tiveram o seu início com Brånemark em meados dos anos 1970. A partir daí, as ligas metálicas, desenho e técnicas evoluíram muito, porém, tanto desenho quanto técnicas de uso de alguns Implantes fogem do que hoje temos como "padrão ouro" dentro da Implantodontia. No presente trabalho, foi relatado um caso clínico de fratura de um implante tipo Garbaccio na região de assoalho nasal, que possui um desenho próprio, com problemas biomecânicos nítidos apesar de ainda ser comercializado. Sendo removido o remanescente do implante fraturado e o paciente reabilitado por um implante com os padrões atuais tipo Brånemark.

Utilização do osso alógeno em bloco para aumento de rebordo alveolar: revisão da literatura / The use of allogeneic bone block for alveolar ridge augmentation: a literature review

Apesar da segurança proporcionada pelo osso autógeno para a correção de defeitos alveolares é constante a busca de alternativas que eliminem ou diminuam a morbidade de uma segunda região operada. Com o advento das técnicas de regeneração óssea guiada é também crescente o interesse pelo uso do osso alógeno. Este trabalho tem por objetivo fazer uma revisão da literatura sobre o uso do osso alógeno em bloco para a reconstrução de rebordos alveolares deficientes, associado ao tratamento com implantes dentários.

Prevalência da xerostomia relacionada à medicação nos pacientes atendidos na área de Odontologia da UNIVILLE / Xerostomy prevalence related to medication on patients assisted by the dentistry area of the UNIVILLE

Este trabalho tem por objetivo determinar a prevalência da xerostomia nos pacientes que procuraram atendimento na Área de Odontologia da UNIVILLE e sua relação com medicamentos utilizados no tratamento de doenças como hipertensão, convulsão, depressão e outros. A metodologia consistiu em fornecer aos pacientes um questionário sobre as condições de saúde geral, sintoma de xerostomia e uso de medicamentos. Os pacientes foram separados e analisados de acordo com o medicamento utilizado no tratamento das doenças, bem como o sintoma isoladamente. De todos os pacientes atendidos, 24,8% relataram xerostomia. Dos que fazem uso de medicamentos o sintoma esteve associado a 35,9%. Os principais medicamentos relacionados ao sintoma nesse estudo foram anti-hipertensivos, antidepressivos e anticonvulsivantes. A xerostomia tem maior prevalência nos pacientes que fazem uso dos medicamentos mencionados

The aim of this research was to know the xerostomy prevalence on patients who have been assisted by the Dentistry Area of the Univille and its relation with some drugs for the following treatments: arterial hypertension disease, seizure, depression and other diseases which require medication. The methodology consists of bringing a questionnaire referent to patients' health conditions, xerostomy symptoms and medications use. Patients were separated and analyzed depending on the medication drug used for their disease treatment and patients presenting isolated xerostomy. From all studied patients,24.8% of them were related with xerostomy. Patients who were relatedto drug medication use presented a 35.9% of this symptom. The main medications related to this xerostomy on this research were: antidepressives, antihypertensives and antiseizure. Xerostomy has a greater prevalence on patients who use the mentioned medications

Schwannoma of the oral cavity: histological and immunohistochemical features / Schwannoma da cavidade bucal: aspectos histológicos e imunohistoquímicos

Schwannoma is a relatively uncommon, slowly growing lesion that is most commonly encountered in the nerve sheath. The mobile portion of the tongue is the most common site, followed by the palate, floor of mouth, buccal mucosa, lips, and jaws. The present case report refere a 13-year-old boy with a tongue mass that did not interfere with the speech. The histopathology and immunohistochemistry study of the excised lesion showed a Schwannoma of the tongue

Avaliaçäo radiográfica da posiçäo de terceiros molares inferiores segundo as classificaçöes de Pell & Gregory e Winter / Radiographic evaluation of lower third molar position according to Pell & Gregory and Winter's classification

A extraçäo de terceiros molares é o procedimento mais comum na prática da cirurgia bucomaxilofacial. A classificaçäo das alteraçöes de posiçäo dos terceiros molares facilita tanto a comunicaçäo entre profissionais como o planejamento cirúrgico das exodontias desses dentes. O objetivo deste trabalho foi classificar, radiograficamente, terceiros molares inferiores de pacientes submetidos à exodontia no Centro Cirúrgico da FOP - Unicampo. Foram examinadas 277 radiografias panorâmicas constantes dos prontuários analisados. Na amostra de 507 terceiros molares inferiores, encontrou-se maior prevalência na posiçäo mesioangular, classe II - B. Os autores enfantizam a necessidade do planejamento pré-cirúrgico com o objetivo de diminuir o risco de acidentes e complicaçöes

Displasia óssea florida / Florid osseous dysplasia

A displasia cemento-óssea florida afeta preferencialmente os ossos maxilares de mulheres negras, acima dos quarenta anos. Esta patologia é caracterizada por ser assintomática e radiograficamente apresentar-se como uma massa radiopaca difusa, geralmente bilateral, podendo estar presente tanto na maxila quanto na mandíbula. Quando infectada, apresenta-se sintomática e necessita de tratamento cirúrgico. O objetivo deste trabalho é discutir as características clínicas, radiográficas, histológicas e a conduta cirúrgica adotada